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overlay agar  (Thermo Fisher)


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    Structured Review

    Thermo Fisher overlay agar
    Overlay Agar, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/overlay+agar/LB+Broth/pmc13085073-64-28-39
    Average 98 stars, based on 1 article reviews
    overlay agar - by Bioz Stars, 2026-10
    98/100 stars

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    Related Articles

    Centrifugation:

    Article Title: Insights into the role of root exudates in bacteriophage infection dynamics
    Article Snippet: To boost phage titer, subsequent phage propagations for bioassays utilized a modified technique by using LB Miller medium (Tryptone 10g/L, Yeast Extract 5g/L, NaCl 10g/L, pH 7.2; Growcells MBLE-7030) instead of BD nutrient broth and by mixing 100 μl of host and phage stock directly into melted overlay agar which was then incubated overnight. .. Phages were harvested by disrupting the overlay agar with 6 ml of SM buffer (50 mM Tris-HCl, 8 mM magnesium sulfate, 100 mM sodium chloride, 0.01% gelatin, pH 7.5; Thermo Scientific, J61367-AK), followed by centrifugation at 8000 g for 10 min and filtration of supernatant via 0.2 μm PES syringe filters. .. The modified technique was used to propagate the gh-1 phage with host P. putida A.3.12 and SPO1 phage (ATCC 27370-B1) with host B. subtilis 168M (ATCC 27370), yielding routinely 3e9 PFU/mL for gh-1 and 5e7 PFU/mL for SPO1.

    Article Title: Virocell Necromass Provides Limited Plant Nitrogen and Elicits Rhizosphere Metabolites That Affect Phage Dynamics.
    Article Snippet: To boost phage titre, subsequent phage propagations for bioassays utilized a modified technique by using LB Miller medium (tryptone 10 g/L, yeast extract 5 g/L, NaCl 10 g/L, pH 7.2; Growcells MBLE‐7030) instead of BD nutrient broth and by mixing 100 μL of host and phage stock directly into melted overlay agar which was then incubated overnight. .. Phages were harvested by disrupting the overlay agar with 6ml of SM buffer (50 mM Tris‐HCl, 8 mM magnesium sulfate, 100mM sodium chloride, 0.01% gelatin, pH 7.5; Thermo Scientific, J61367‐AK), followed by centrifugation at 8000g for 10 min and filtration of supernatant via 0.2 μm PES syringe filters. .. The modified technique was used to propagate the gh‐1 phage with host P. putida A.3.12 and SPO1 phage (ATCC 27370‐B1) with host B. subtilis 168M (ATCC 27370), yielding routinely 3 × 109 PFU/mL for gh‐1 and 5 × 107 PFU/mL for SPO1.

    Filtration:

    Article Title: Insights into the role of root exudates in bacteriophage infection dynamics
    Article Snippet: To boost phage titer, subsequent phage propagations for bioassays utilized a modified technique by using LB Miller medium (Tryptone 10g/L, Yeast Extract 5g/L, NaCl 10g/L, pH 7.2; Growcells MBLE-7030) instead of BD nutrient broth and by mixing 100 μl of host and phage stock directly into melted overlay agar which was then incubated overnight. .. Phages were harvested by disrupting the overlay agar with 6 ml of SM buffer (50 mM Tris-HCl, 8 mM magnesium sulfate, 100 mM sodium chloride, 0.01% gelatin, pH 7.5; Thermo Scientific, J61367-AK), followed by centrifugation at 8000 g for 10 min and filtration of supernatant via 0.2 μm PES syringe filters. .. The modified technique was used to propagate the gh-1 phage with host P. putida A.3.12 and SPO1 phage (ATCC 27370-B1) with host B. subtilis 168M (ATCC 27370), yielding routinely 3e9 PFU/mL for gh-1 and 5e7 PFU/mL for SPO1.

    Article Title: Virocell Necromass Provides Limited Plant Nitrogen and Elicits Rhizosphere Metabolites That Affect Phage Dynamics.
    Article Snippet: To boost phage titre, subsequent phage propagations for bioassays utilized a modified technique by using LB Miller medium (tryptone 10 g/L, yeast extract 5 g/L, NaCl 10 g/L, pH 7.2; Growcells MBLE‐7030) instead of BD nutrient broth and by mixing 100 μL of host and phage stock directly into melted overlay agar which was then incubated overnight. .. Phages were harvested by disrupting the overlay agar with 6ml of SM buffer (50 mM Tris‐HCl, 8 mM magnesium sulfate, 100mM sodium chloride, 0.01% gelatin, pH 7.5; Thermo Scientific, J61367‐AK), followed by centrifugation at 8000g for 10 min and filtration of supernatant via 0.2 μm PES syringe filters. .. The modified technique was used to propagate the gh‐1 phage with host P. putida A.3.12 and SPO1 phage (ATCC 27370‐B1) with host B. subtilis 168M (ATCC 27370), yielding routinely 3 × 109 PFU/mL for gh‐1 and 5 × 107 PFU/mL for SPO1.

    Isolation:

    Article Title: Diverse bacteriophages for biocontrol of ESBL- and AmpC-β-lactamase-producing E. coli
    Article Snippet: .. Depending on the size of the Petri dish used, the bacterial lawns were prepared from 100 or 300 μl overnight cultures of isolation strains that were mixed with 4 or 10 ml of molten overlay agar (LBov; LB broth with 0,6% Agar bacteriological no.1 (Oxoid)) and spread on 9 or 12 cm LA (LB with 1,2% agar) plates, respectively. ..

    Spot Test:

    Article Title: Heterologous expression and antimicrobial potential of class II bacteriocins.
    Article Snippet: Strains with Biosafety level 2 were handled in the same manner, except that a Coy Laboratory Products Vinyl; gas mixture, 95% N2 and 5% H2 was used to make anaerobic conditions. .. Overlay agar for spot assay For each square plate (Thermo fisher scientific omnitray w/lid Non treated sterile #264728) in total of 12 ml pre-reduced top agar was used (0.5% agar) (Milipore # 69964) +100 μl of the target strain adjusted to OD ~ 0.5. ..

    Sterility:

    Article Title: Heterologous expression and antimicrobial potential of class II bacteriocins.
    Article Snippet: Strains with Biosafety level 2 were handled in the same manner, except that a Coy Laboratory Products Vinyl; gas mixture, 95% N2 and 5% H2 was used to make anaerobic conditions. .. Overlay agar for spot assay For each square plate (Thermo fisher scientific omnitray w/lid Non treated sterile #264728) in total of 12 ml pre-reduced top agar was used (0.5% agar) (Milipore # 69964) +100 μl of the target strain adjusted to OD ~ 0.5. ..



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